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This Article Full Text Full Text (PDF) All Versions of this Article: 40/3/189    most recent 00120.2009v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Rudkowska, I. Articles by Vohl, M.-C. PubMed PubMed Citation Articles by Rudkowska, I. Articles by Vohl, M.-C.

Research Articles

Fish nutrients decrease expression levels of tumor necrosis factor- in cultured human macrophages

Lipid Research Center, CHUL Research Center, and Nutraceuticals and Functional Foods Institute (INAF), Laval University, Quebec City, Quebec, Canada

Numerous studies have demonstrated the beneficial effects of fish consumption on inflammatory markers. Until now, these beneficial effects of fish consumption have been mostly linked to the omega-3 fatty acids (FA). The objective of the present study was to examine, in vitro, whether expression levels of genes involved in the inflammatory response differ in human macrophages incubated with casein hydrolysates (CH) or fish protein hydrolysates (FPH) in the presence or absence of omega-3 FA compared with omega-3 FA alone. Peripheral blood monocytes differentiated into macrophages from 10 men were incubated in the presence of omega-3 FA (10 µM eicosapentaenoic acid and 5 µM docosahexaenoic acid) or CH or FPH (10, 100, 1,000 µg) with or without omega-3 FA for 48 h. Results demonstrate that expression levels of tumor necrosis factor (TNF) had a tendency to be lower after the addition of FPH alone or CH with omega-3 FA compared with omega-3 FA treatment. Furthermore, the combination of FPH and omega-3 FA synergistically decreased expression levels of TNF compared to treatment with omega-3 FA or FPH alone. No difference on gene expression levels of interleukin-6 was observed between treatments. In conclusion, these preliminary results suggest that the anti-inflammatory effects of fish consumption can be explained by a synergistic effect of the omega-3 FA with the protein components of fish on TNF expression and therefore contribute to the beneficial effects of fish consumption. Hence, follow-up studies should be performed to confirm the effects of a diet rich in FPH and omega-3 FA on serum proinflammatory cytokine concentrations.

omega-3 fatty acids; fish protein; inflammation; interleukin-6