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Physiol. Genomics 4: 75-81, 2000;
1094-8341/00 $5.00
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Received 26 July 2000; accepted in final form 10 September 2000.
Physiological Genomics 4:75-81 (2000)
1094-8341/00 $5.00 © 2000 American Physiological Society

Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues

C. A. JONES1, M. I. HURLEY2, T. A. BLACK3, C. M. KANE1, L. PAN1, S. C. PRUITT1 and K. W. GROSS1

1 Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo 14260
2 Department of Mathematics and Natural Sciences, D’Youville College, Buffalo, New York 14201

A reporter construct was assembled with 4-kb of renin 5'-flanking sequence fused to humanized green fluorescent protein (GFP) cDNA. Transgenic mice carrying this construct were produced and assayed for GFP expression. In the adult, expression was detected in juxtaglomerular (JG) cells of the kidney and granular convoluted tubular cells of the submandibular gland. Furthermore, treatment of mice with captopril induced GFP expression in renal vascular smooth muscle cells. During embryogenesis, GFP expression was first detected at embryonic day E13 in the adrenal gland and Wolffian duct. Expression was also seen in the developing renal vasculature as early as E14 and remained detectable through birth. Renal GFP expression became restricted to JG cells in adults. Fetal adrenal and gonadal arteries also expressed GFP. In the placenta, GFP was observed in giant cell trophoblasts, consistent with reports of renin expression in chorionic cells of both humans and mice. We conclude that 4 kb of renin 5' flank is sufficient to direct multiple known renin expression patterns. Furthermore, the renin-GFP construct characterized here will provide a useful vital reporter for renin expression.

reporter gene; green fluorescent protein; giant cell trophoblast; juxtaglomerular cells; placenta




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