Physiol. Genomics AJP: Advances in Physiology Education
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Physiol. Genomics 38: 7-15, 2009. First published March 17, 2009; doi:10.1152/physiolgenomics.90287.2008
1094-8341/09 $8.00
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Received 10 July 2008; accepted in final form 12 March 2009.
Physiological Genomics 38:7-15 (2009)
1094-8341/09 $8.00 © 2009 American Physiological Society

Call For Papers: Comparative Genomics

Genomic profiling of developing cardiomyocytes from recombinant murine embryonic stem cells reveals regulation of transcription factor clusters

Michael J. Seewald 1,*, Peter Ellinghaus 1,*, Astrid Kassner 2, Ines Stork 2, Martina Barg 1, Sylvia Niebrügge 3, Stefan Golz 1, Holger Summer 1, Robert Zweigerdt 4, Eva-Maria Schräder 2, Samantha Feicht 2, Kornelia Jaquet 5, Stephanie Reis 5, Reiner Körfer 2 and Hendrik Milting 2

1 Department of Target Discovery, Bayer Healthcare AG, Wuppertal; 2 Herz- und Diabeteszentrum Nordrhein-Westfalen, Erich und Hanna Klessmann-Institut für Kardiovaskuläre Forschung und Entwicklung, Bad Oeynhausen, Germany; 3 Stem Cell Bioengineering Laboratory; University of Toronto; Terrence Donnelly Centre for Cellular and Biomolecular Research; Toronto, Ontario, Canada; 4 Institute of Medical Biology, Singapore; and 5 Abteilung für Molekulare Kardiologie, Berufsgenossenschaftliche Kliniken Bergmannsheil, Bochum, Germany

Cardiomyocytes derived from pluripotent embryonic stem cells (ESC) have the advantage of providing a source for standardized cell cultures. However, little is known on the regulation of the genome during differentiation of ESC to cardiomyocytes. Here, we characterize the transcriptome of the mouse ESC line CM7/1 during differentiation into beating cardiomyocytes and compare the gene expression profiles with those from primary adult murine cardiomyocytes and left ventricular myocardium. We observe that the cardiac gene expression pattern of fully differentiated CM7/1-ESC is highly similar to adult primary cardiomyocytes and murine myocardium, respectively. This finding is underlined by demonstrating pharmacological effects of catecholamines and endothelin-1 on ESC-derived cardiomyocytes. Furthermore, we monitor the temporal changes in gene expression pattern during ESC differentiation with a special focus on transcription factors involved in cardiomyocyte differentiation. Thus, CM7/1-ESC-derived cardiomyocytes are a promising new tool for functional studies of cardiomyocytes in vitro and for the analysis of the transcription factor network regulating pluripotency and differentiation to cardiomyocytes.

microarrays; differentiation; comparative transcriptome






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