Physiol. Genomics Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 37: 35-42, 2009. First published December 30, 2008; doi:10.1152/physiolgenomics.00051.2008
1094-8341/09 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Video
Right arrow All Versions of this Article:
37/1/35    most recent
00051.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pacheco-Pinedo, E. C.
Right arrow Articles by Khurana, T. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pacheco-Pinedo, E. C.
Right arrow Articles by Khurana, T. S.
Received 11 March 2008; accepted in final form 23 December 2008.
Physiological Genomics 37:35-42 (2009)
1094-8341/09 $8.00 © 2009 American Physiological Society

Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles

Eugenia C. Pacheco-Pinedo 1,2,*, Murat T. Budak 2,3,*, Ulrike Zeiger 1,2, Louise Helskov Jørgensen 4,5, Sasha Bogdanovich 1,2, Henrik Daa Schrøder 5, Neal A. Rubinstein 2,3 and Tejvir S. Khurana 1,2

1 Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania
2 Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania
3 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania
4 Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
5 Department of Clinical Pathology, University of Southern Denmark, Odense, Denmark

The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in previous whole muscle microarray studies. Cultured EOM-SP cells revealed superior in vitro proliferative capacity. Finally, assays of the committed progenitors or satellite cells performed on myofibers isolated from EOM and limb muscles independently validated the increased proliferative capacity of these muscles. We suggest a model in which unique EOM stem cells contribute to the continual myogenesis noted in EOM and consistent with a role for their sparing in DMD. We believe the greater numbers of stem cells, their unique transcriptome, the greater proliferative capacity of EOM stem cells, and the greater number of satellite cells also offer clues for novel cell-based therapeutic strategies.

side population; microarrays; Duchenne muscular dystrophy






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2009 by the American Physiological Society.