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This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 36/3/149    most recent 90216.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Smith, I. J. Articles by Kraus, W. E. Search for Related Content PubMed PubMed Citation Articles by Smith, I. J. Articles by Kraus, W. E.

Sex-specific alterations in mRNA level of key lipid metabolism enzymes in skeletal muscle of overweight and obese subjects following endurance exercise

¹ Center for the Study of Aging, Veterans Affairs Medical Center, Durham, North Carolina
² Division of Cardiovascular Medicine, Veterans Affairs Medical Center, Durham, North Carolina
³ Division of Rheumatology, Department of Medicine, Veterans Affairs Medical Center, Durham, North Carolina
⁴ Duke Center for Living, Veterans Affairs Medical Center, Durham, North Carolina
⁵ School of Medicine, Duke University Medical Center, Veterans Affairs Medical Center, Durham, North Carolina
⁶ Physical Medicine and Rehabilitation Service, Veterans Affairs Medical Center, Durham, North Carolina

Endurance exercise (EE) leads to beneficial alterations in skeletal muscle lipid metabolism in overweight and obese individuals; however, the mechanisms of these improvements are poorly understood. The primary goal of the current investigation was to test the hypothesis that long-term EE training (6 mo) leads to alterations in the mRNA abundance of key lipid metabolism enzymes in skeletal muscle of overweight and obese middle-aged women and men. A secondary aim of this study was to investigate the hypothesis that exercise-mediated adaptations in mRNA levels differ between women and men. The mRNA abundance of representative lipogenic and lipolytic genes from major lipid metabolism pathways, as well as representative lipogenic and lipolytic transcription factors, were determined by real-time PCR from skeletal muscle biopsies collected before and 24 h after the final bout of 6 mo of EE. Six months of EE led to increases in muscle lipoprotein lipase, peroxisome proliferator-activated receptor- coactivator-1, carnitine palmitoyltransferase-1 β, diacylglycerol acyltransferase-1, and acid ceramidase mRNA in women, but not men. In contrast, in men, EE led to reductions in the mRNA content of the lipogenic factors sterol regulatory element binding protein-1c and serine palmitoyl transferase. These data suggest that EE-mediated alterations in the abundance of the lipid metabolism genes studied here are fundamentally different between overweight and obese middle-aged women and men. Future studies should determine whether these adaptations in mRNA levels translate into changes in protein function.

obesity; metabolic syndrome; diabetes; insulin resistance; cardiovascular disease