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Physiol. Genomics 35: 283-295, 2008. First published August 26, 2008; doi:10.1152/physiolgenomics.90213.2008
1094-8341/08 $8.00
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Received 8 April 2008; accepted in final form 26 August 2008.
Physiological Genomics 35:283-295 (2008)
1094-8341/08 $8.00 © 2008 American Physiological Society

Effect of starvation on transcriptomes of brain and liver in adult female zebrafish (Danio rerio)

Robert E. Drew 1, Kenneth J. Rodnick 2, Matthew Settles 3, Jurij Wacyk 4, Erin Churchill 1, Madison S. Powell 5, Ronald W. Hardy 5, Gordon K. Murdoch 4, Rodney A. Hill 4 and Barrie D. Robison 1

1 Department of Biological Sciences and Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow
2 Department of Biological Sciences, Idaho State University, Pocatello
3 Department of Computer Science and Program in Bioinformatics and Computational Biology, University of Idaho, Moscow
4 Animal and Veterinary Sciences, University of Idaho, Moscow
5 Hagerman Fish Culture Experiment Station, University of Idaho, Hagerman, Idaho

We used microarray and quantitative real-time PCR (qRT-PCR) analyses in adult female zebrafish (Danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. The transcriptome of whole zebrafish brain showed little response to 21 days of starvation. Only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. In contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcripts in the liver, indicating an overall decrease in metabolic activity, reduced lipid metabolism, protein biosynthesis, proteolysis, and cellular respiration, and increased gluconeogenesis. Starvation also regulated expression of many components of the unfolded protein response, the first such report in a species other than yeast (Saccharomyces cerevisiae) and mice (Mus musculus). The response of the zebrafish hepatic transcriptome to starvation was strikingly similar to that of rainbow trout (Oncorhynchus mykiss) and less similar to mouse, while the response of common carp (Cyprinus carpio) differed considerably from the other three species.

microarray analysis; metabolic signaling; quantitative real-time polymerase chain reaction; neuropeptide






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