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Physiol. Genomics 34: 256-264, 2008. First published June 10, 2008; doi:10.1152/physiolgenomics.90234.2008
1094-8341/08 $8.00
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Received 1 May 2008; accepted in final form 4 June 2008.
Physiological Genomics 34:256-264 (2008)
1094-8341/08 $8.00 © 2008 American Physiological Society

Call For Papers: Comparative Genomics

Functional and structural characterization of the zebrafish Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1)

Daniel Markovich1, Alessandro Romano2, Carlo Storelli2 and Tiziano Verri2

1 Molecular Biology Lab, School of Biomedical Sciences, University of Queensland, St. Lucia, Australia
2 Department of Biological and Environmental Sciences and Technologies, University of Salento (formerly University of Lecce), Lecce, Italy

Sulfate plays an essential role during growth, development and cellular metabolism. In this study, we characterized the function and structure of the zebrafish (Danio rerio) Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1). Zebrafish NaS1 encodes a protein of 583 amino acids with 13 putative transmembrane domains. Expression of zebrafish NaS1 protein in Xenopus oocytes led to Na+-sulfate cotransport, which was significantly inhibited by thiosulfate, selenate, molybdate, and tungstate. Zebrafish NaS1 transport kinetics were: Vmax = 1,731.670 ± 92.853 pmol sulfate/oocyte·hour and Km = 1.414 ± 0.275 mM for sulfate and Vmax = 307.016 ± 32.992 pmol sulfate/oocyte·hour, Km = 24.582 ± 4.547 mM and n (Hill coefficient) = 1.624 ± 0.354 for sodium. Zebrafish NaS1 mRNA is developmentally expressed in embryos from day 1 postfertilization and in the intestine, kidney, brain, and eye of adult zebrafish. The zebrafish NaS1 gene slc13a1 contains 15 exons spanning 8,716 bp. Characterization of the zebrafish NaS1 contributes to a greater understanding of sulfate transporters in a well-defined genetic model and will allow the elucidation of evolutionary and functional relationships among vertebrate sulfate transporters.

sulfate; transporter; gene expression; Xenopus laevis oocytes






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