Physiol. Genomics Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 33: 193-204, 2008. First published February 19, 2008; doi:10.1152/physiolgenomics.00274.2007
1094-8341/08 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
33/2/193    most recent
00274.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Forde, N.
Right arrow Articles by Evans, A. C. O.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Forde, N.
Right arrow Articles by Evans, A. C. O.
Received 21 November 2007; accepted in final form 15 February 2008.
Physiological Genomics 33:193-204 (2008)
1094-8341/08 $8.00 © 2008 American Physiological Society

Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle

N. Forde1, M. Mihm2, M. J. Canty1, A. E. Zielak1, P. J. Baker2, S. Park1, P. Lonergan1, G. W. Smith3, P. M. Coussens3, J. J. Ireland3 and A. C. O. Evans1

1 School of Agriculture Food Science and Veterinary Medicine and the Conway Institute for Biomolecular and Biomedical Research, College of Life Sciences, University College Dublin, Belfield, Dublin, Ireland
2 Division of Cell Sciences, Faculty of Veterinary Medicine, University of Glasgow, Glasgow, United Kingdom
3 Department of Animal Science and Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan

Ovarian follicles develop in groups yet individual follicles follow different growth trajectories. This growth and development are regulated by endocrine and locally produced growth factors that use a myriad of receptors and signal transduction pathways to exert their effects on theca and granulosa cells. We hypothesize that differential growth may be due to differences in hormonal responsiveness that is partially mediated by differences in expression of genes involved in signal transduction. We used the bovine dominant follicle model, microarrays, quantitative real-time PCR and RNA interference to examine this. We identified 83 genes coding for signal transduction molecules and validated a subset of them associated with different stages of the follicle wave. We suggest important roles for CAM kinase-1 and EphA4 in theca cells and BCAR1 in granulosa cells for the development of dominant follicles and for betaglycan and FIBP in granulosa cells of regressing subordinate follicles. Inhibition of genes for betaglycan and FIBP in granulosa cells in vitro suggests that they inhibit estradiol production in regressing subordinate follicles.

signaling; RNA interference; fibroblast growth factor intracellular binding protein






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2008 by the American Physiological Society.