Physiol. Genomics AJP: Regulatory, Integrative and Comparative Physiology
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Physiol. Genomics 33: 110-120, 2008. First published January 15, 2008; doi:10.1152/physiolgenomics.00251.2007
1094-8341/08 $8.00
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Received 25 October 2007; accepted in final form 11 January 2008.
Physiological Genomics 33:110-120 (2008)
1094-8341/08 $8.00 © 2008 American Physiological Society

Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride

Valerio Del Vescovo 1,*, Viviana Casagrande 1,*, Michele M. Bianchi 1, Eugenia Piccinni 1, Laura Frontali 1,4, Cristina Militti 1, Vivienne Fardeau 2, Frédéric Devaux 2, Claudio Di Sanza 3, Carlo Presutti 3,4 and Rodolfo Negri 1,4

1 Istituto Pasteur Fondazione Cenci-Bolognetti, Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Roma, La Sapienza, Rome, Italy
2 Laboratoire de Génomique CNRS, Ecole Normale Supérieure, Paris, France
3 Dipartimento di Genetica e Biologia Molecolare, Università degli Studi di Roma, La Sapienza
4 Laboratory of Functional Genomics and Proteomics of Model Systems, University of Rome, La Sapienza, Rome, Italy

We analyzed the global transcriptional response of Saccharomyces cerevisiae cells exposed to different concentrations of CsCl in the growth medium and at different times after addition. Early responsive genes were mainly involved in cell wall structure and biosynthesis. About half of the induced genes were previously shown to respond to other alkali metal cations in a Hog1-dependent fashion. Western blot analysis confirmed that cesium concentrations as low as 100 mM activate Hog1 phosphorylation. Another important fraction of the cesium-modulated genes requires Yaf9p for full responsiveness as shown by the transcriptome of a yaf9-deleted strain in the presence of cesium. We showed that a cell wall-restructuring process promptly occurs in response to cesium addition, which is dependent on the presence of both Hog1 and Yaf9 proteins. Moreover, the sensitivity to low concentration of cesium of the yaf9-deleted strain is not observed in a strain carrying the hog1/yaf9 double deletion. We conclude that the observed early transcriptional modulation of cell wall genes has a crucial role in S. cerevisiae adaptation to cesium.

caesium; alkali metal cations; cell wall; YAF9; HOG1






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