Physiol. Genomics Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 30: 192-204, 2007. First published April 10, 2007; doi:10.1152/physiolgenomics.00198.2006
1094-8341/07 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
30/2/192    most recent
00198.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bünger, M.
Right arrow Articles by Müller, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bünger, M.
Right arrow Articles by Müller, M.
Received 11 September 2006; accepted in final form 3 April 2007.
Physiological Genomics 30:192-204 (2007)
1094-8341/07 $8.00 © 2007 American Physiological Society

Genome-wide analysis of PPAR{alpha} activation in murine small intestine

Meike Bünger 1,2, Heleen M. van den Bosch 1,2, Jolanda van der Meijde 1, Sander Kersten 1,2, Guido J. E. J. Hooiveld 1,2 and Michael Müller 1,2

1 Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University
2 Nutrigenomics Consortium, Wageningen Centre for Food Sciences, Wageningen, the Netherlands

The peroxisome proliferator-activated receptor alpha (PPAR{alpha}) is a fatty acid-activated transcription factor that governs a variety of biological processes. Little is known about the role of PPAR{alpha} in the small intestine. Since this organ is frequently exposed to high levels of PPAR{alpha} ligands via the diet, we set out to characterize the function of PPAR{alpha} in small intestine using functional genomics experiments and bioinformatics tools. PPAR{alpha} was expressed at high levels in both human and murine small intestine. Detailed analyses showed that PPAR{alpha} was expressed most highly in villus cells of proximal jejunum. Microarray analyses of total tissue samples revealed, that in addition to genes involved in fatty acid and triacylglycerol metabolism, transcription factors and enzymes connected to sterol and bile acid metabolism, including FXR and SREBP1, were specifically induced. In contrast, genes involved in cell cycle and differentiation, apoptosis, and host defense were repressed by PPAR{alpha} activation. Additional analyses showed that intestinal PPAR{alpha}-dependent gene regulation occurred in villus cells. Functional implications of array results were corroborated by morphometric data. The repression of genes involved in proliferation and apoptosis was accompanied by a 22% increase in villus height and a 34% increase in villus area of wild-type animals treated with WY14643. This is the first report providing a comprehensive overview of processes under control of PPAR{alpha} in the small intestine. We show that PPAR{alpha} is an important transcriptional regulator in small intestine, which may be of importance for the development of novel foods and therapies for obesity and inflammatory bowel diseases.

microarray; peroxisome proliferator-activated receptor alpha; gene expression; crypt-villus axis; lipid absorption; proximal-distal axis




This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
Y. Yamamoto, T. Tanahashi, T. Kawai, S. Chikahisa, S. Katsuura, K. Nishida, S. Teshima-Kondo, H. Sei, and K. Rokutan
Changes in behavior and gene expression induced by caloric restriction in C57BL/6 mice
Physiol Genomics, November 1, 2009; 39(3): 227 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
P. J. H. Smeets, H. M. de Vogel-van den Bosch, P. H. M. Willemsen, A. P. Stassen, T. Ayoubi, G. J. van der Vusse, and M. van Bilsen
Transcriptomic analysis of PPAR{alpha}-dependent alterations during cardiac hypertrophy
Physiol Genomics, December 12, 2008; 36(1): 15 - 23.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Colin, E. Bourguignon, A.-B. Boullay, J.-J. Tousaint, S. Huet, F. Caira, B. Staels, S. Lestavel, J.-M. A. Lobaccaro, and P. Delerive
Intestine-Specific Regulation of PPAR{alpha} Gene Transcription by Liver X Receptors
Endocrinology, October 1, 2008; 149(10): 5128 - 5135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Fu, J. Kim, F. Oveisi, G. Astarita, and D. Piomelli
Targeted enhancement of oleoylethanolamide production in proximal small intestine induces across-meal satiety in rats
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R45 - R50.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. de Wilde, R. Mohren, S. van den Berg, M. Boekschoten, K. W.-V. Dijk, P. de Groot, M. Muller, E. Mariman, and E. Smit
Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice
Physiol Genomics, February 19, 2008; 32(3): 360 - 369.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.