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1 Brigham and Women’s Hospital, Department of Medicine, Harvard Institutes of Medicine, Boston, Massachusetts 02115
2 Genisphere, Philadelphia, Pennsylvania 19131
Stears, Robin L., Robert C. Getts, and Steven R. Gullans. A novel, sensitive detection system for high-density microarrays using dendrimer technology. Physiol Genomics 3: 93–99, 2000.—To improve signal detection on cDNA microarrays, we adapted a fluorescent oligonucleotide dendrimeric signal amplification system to microarray technology. This signal detection method requires 16-fold less RNA for probe synthesis, does not depend on the incorporation of fluorescent dNTPs into a reverse transcription reaction, generates a high signal-to-background ratio, and can be used to allow for multichannel detection on a single chip. Furthermore, since the dendrimers can be detected individually, it may be possible, by employing dendrimer-binding standards, to calculate the numbers of bound cDNAs can be estimated. These features make the dendrimer signal detection reagent ideal for high-throughput functional genomics research.
high-density DNA microarrays; dendrimers; signal detection
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