Physiol. Genomics Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 3: 75-82, 2000;
1094-8341/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SHI, H.
Right arrow Articles by ZEMEL, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SHI, H.
Right arrow Articles by ZEMEL, M. B.
Received 7 February 2000; accepted in final form 29 May 2000.
Physiological Genomics 3:75-82 (2000)
1094-8341/00 $5.00 © 2000 American Physiological Society

Role of intracellular calcium in human adipocyte differentiation

HANG SHI1, YUAN-DI HALVORSEN2, PAMELA N. ELLIS1, WILLIAM O. WILKISON2 and MICHAEL B. ZEMEL1

1 Department of Nutrition, The University of Tennessee, Knoxville, Tennessee 37996
2 Zen-Bio, Research Triangle Park, North Carolina 27709

Shi, Hang, Yuan-Di Halvorsen, Pamela N. Ellis, William O. Wilkison, and Michael B. Zemel. Role of intracellular calcium in human adipocyte differentiation. Physiol Genomics 3: 75–82, 2000.—Intracellular calcium ([Ca2+]i) modulates adipocyte lipid metabolism and inhibits the early stages of murine adipogenesis. Consequently, we evaluated effects of increasing [Ca2+]i in early and late stages of human adipocyte differentiation. Increasing [Ca2+]i with either thapsigargin or A23187 at 0–1 h of differentiation markedly suppressed differentiation, with a 40–70% decrease in triglyceride accumulation and glycerol-3 phosphate dehydrogenase (GPDH) activity (P < 0.005). However, a 1-h pulse of either agent at 47–48 h only modestly inhibited differentiation. Sustained, mild stimulation of Ca2+ influx with either agouti protein or 10 mM KCl-induced depolarization during 0–48 h of differentiation inhibited triglyceride accumulation and GPDH activity by 20–70% (P < 0.05) and markedly suppressed peroxisome proliferator-activated receptor gamma (PPAR{gamma}) expression. These effects were reversed by Ca2+ channel antagonism. In contrast, Ca2+ pulses late in differentiation (71–72 h or 48–72 h) markedly increased these markers of differentiation. Thus increasing [Ca2+]i appears to exert a biphasic regulatory role in human adipocyte differentiation, inhibiting the early stages while promoting the late stage of differentiation and lipid filling.

preadipocyte differentiation; intracellular calcium; obesity




This article has been cited by other articles:


Home page
 Circ. Res.Home page
D. L. Cioffi
The Skinny on TRPV1
Circ. Res., April 13, 2007; 100(7): 934 - 936.
[Full Text] [PDF]

Home page
 Circ. Res.Home page
L. L. Zhang, D. Yan Liu, L. Q. Ma, Z. D. Luo, T. B. Cao, J. Zhong, Z. C. Yan, L. J. Wang, Z. G. Zhao, S. J. Zhu, et al.
Activation of Transient Receptor Potential Vanilloid Type-1 Channel Prevents Adipogenesis and Obesity
Circ. Res., April 13, 2007; 100(7): 1063 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
S. N Rajpathak, E. B Rimm, B. Rosner, W. C Willett, and F. B Hu
Calcium and dairy intakes in relation to long-term weight gain in US men
Am. J. Clinical Nutrition, March 1, 2006; 83(3): 559 - 566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
N. Boon, G. B. Hul, N. Viguerie, A. Sicard, D. Langin, and W. H. Saris
Effects of 3 diets with various calcium contents on 24-h energy expenditure, fat oxidation, and adipose tissue message RNA expression of lipid metabolism-related proteins
Am. J. Clinical Nutrition, December 1, 2005; 82(6): 1244 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Am J EpidemiolHome page
N. Boon, L. L. J. Koppes, W. H. M. Saris, and W. Van Mechelen
The Relation between Calcium Intake and Body Composition in a Dutch Population: The Amsterdam Growth and Health Longitudinal Study
Am. J. Epidemiol., July 1, 2005; 162(1): 27 - 32.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
X. Sun and M. B. Zemel
Calcium and Dairy Products Inhibit Weight and Fat Regain during Ad Libitum Consumption Following Energy Restriction in Ap2-Agouti Transgenic Mice
J. Nutr., November 1, 2004; 134(11): 3054 - 3060.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Su, D. J. Mancuso, P. E. Bickel, C. M. Jenkins, and R. W. Gross
Small Interfering RNA Knockdown of Calcium-independent Phospholipases A2 {beta} or {gamma} Inhibits the Hormone-induced Differentiation of 3T3-L1 Preadipocytes
J. Biol. Chem., May 21, 2004; 279(21): 21740 - 21748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. A. Rufo, P. W. Lin, A. Andrade, L. Jiang, L. Rameh, C. Flexner, S. L. Alper, and W. I. Lencer
Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C998 - C1008.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. A. Shapses, S. Heshka, and S. B. Heymsfield
Effect of Calcium Supplementation on Weight and Fat Loss in Women
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 632 - 637.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
M. Jacqmain, E. Doucet, J.-P. Despres, C. Bouchard, and A. Tremblay
Calcium intake, body composition, and lipoprotein-lipid concentrations in adults
Am. J. Clinical Nutrition, June 1, 2003; 77(6): 1448 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Neal and N. A. Clipstone
Calcineurin Mediates the Calcium-dependent Inhibition of Adipocyte Differentiation in 3T3-L1 Cells
J. Biol. Chem., December 13, 2002; 277(51): 49776 - 49781.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. B. Hansen-Petrik, M. F. McEntee, B. Jull, H. Shi, M. B. Zemel, and J. Whelan
Prostaglandin E2 Protects Intestinal Tumors from Nonsteroidal Anti-inflammatory Drug-induced Regression in ApcMin/+ Mice
Cancer Res., January 1, 2002; 62(2): 403 - 408.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online