HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 28/1/84    most recent 00111.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by El-Sayed, A. Articles by Tesfaye, D. Search for Related Content PubMed PubMed Citation Articles by El-Sayed, A. Articles by Tesfaye, D.

Call For Papers: 2nd International Symposium on Animal Functional Genomics

Large-scale transcriptional analysis of bovine embryo biopsies in relation to pregnancy success after transfer to recipients

¹ Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany
² Centre de Recherche en Biologie de la Reproduction, Department of Animal Sciences, Laval University, Pav. Comtois, Sainte-Foy, Quebec, Canada

ABSTRACT

The purpose of this work is to address the relationship between transcriptional profile of embryos and the pregnancy success based on gene expression analysis of blastocyst biopsies taken prior to transfer to recipients. Biopsies (30–40% of the intact embryo) were taken from in vitro-produced day 7 blastocysts (n = 118), and 60–70% were transferred to recipients after reexpansion. Based on the success of pregnancy, biopsies were pooled in three groups (each 10 biopsies) namely: those resulting in no pregnancy (G1), resorbed embryos (G2), and those resulting in calf delivery (G3). Gene expression analysis of these groups was performed using home-made bovine preimplantation-specific cDNA array (219 clones) and BlueChip (with 2,000 clones). Microarray data analysis results revealed a total of 52 and 58 genes were differentially regulated during comparison between G1 vs. G3 and G2 vs. G3. Biopsies resulted in calf delivery were enriched with genes necessary for implantation (COX2 and CDX2), carbohydrate metabolism (ALOX15), growth factor (BMP15), signal transduction (PLAU), and placenta-specific 8 (PLAC8). Biopsies from embryos resulting in resorption are enriched with transcripts involved protein phosphorylation (KRT8), plasma membrane (OCLN), and glucose metabolism (PGK1 and AKR1B1). Biopsies from embryos resulting in no pregnancy are enriched with transcripts involved inflammatory cytokines (TNF), protein amino acid binding (EEF1A1), transcription factors (MSX1, PTTG1), glucose metabolism (PGK1, AKR1B1), and CD9, which is an inhibitor of implantation. In conclusion, we generated direct candidates of blastocyst-specific genes which may play an important role in determining the fate of the embryo after transfer.

blastocyst; preimplantation; embryo loss; microarray

This article has been cited by other articles:

				S L Rodriguez-Zas, K Schellander, and H A Lewin Biological interpretations of transcriptomic profiles in mammalian oocytes and embryos Reproduction, February 1, 2008; 135(2): 129 - 139. [Abstract] [Full Text] [PDF]

				V. Duranthon, A. J Watson, and P. Lonergan Preimplantation embryo programming: transcription, epigenetics, and culture environment Reproduction, February 1, 2008; 135(2): 141 - 150. [Abstract] [Full Text] [PDF]

				H. Torner, N. Ghanem, C. Ambros, M. Holker, W. Tomek, C. Phatsara, H. Alm, M.-A. Sirard, W. Kanitz, K. Schellander, et al. Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity Reproduction, February 1, 2008; 135(2): 197 - 212. [Abstract] [Full Text] [PDF]

				J. L. Burton and G. J. M. Rosa Physiological genomics special issue on animal functional genomics Physiol Genomics, December 13, 2006; 28(1): 1 - 4. [Full Text] [PDF]