HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 27/3/328    most recent 00090.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Martyniuk, C. J. Articles by Trudeau, V. L. Search for Related Content PubMed PubMed Citation Articles by Martyniuk, C. J. Articles by Trudeau, V. L.

Gene expression profiling in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17-ethinylestradiol

Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

17- Ethinylestradiol (EE2), a pharmaceutical estrogen, is detectable in water systems worldwide. Although studies report on the effects of xenoestrogens in tissues such as liver and gonad, few studies to date have investigated the effects of EE2 in the vertebrate brain at a large scale. The purpose of this study was to develop a goldfish brain-enriched cDNA array and use this in conjunction with a mixed tissue carp microarray to study the genomic response to EE2 in the brain. Gonad-intact male goldfish were exposed to nominal concentrations of 0.1 nM (29.6 ng/l) and 1.0 nM (296 ng/l) EE2 for 15 days. Male goldfish treated with the higher dose of EE2 had significantly smaller gonads compared with controls. Males also had a significantly reduced level of circulating testosterone (T) and 17ß-estradiol (E2) in both treatment groups. Candidate genes identified by microarray analysis fall into functional categories that include neuropeptides, cell metabolism, and transcription/translation factors. Differentially expressed genes verified by real-time RT-PCR included brain aromatase, secretogranin-III, and interferon-related developmental regulator 1. Our results suggest that the expression of genes in the sexually mature adult brain appears to be resistant to low EE2 exposure but is affected significantly at higher doses of EE2. This study demonstrates that microarray technology is a useful tool to study the effects of endocrine disrupting chemicals on neuroendocrine function and suggest that exposure to EE2 may have significant effects on localized E2 synthesis in the brain by affecting transcription of brain aromatase.

microarray; hypothalamus; aromatase; endocrine disruption

This article has been cited by other articles:

				D. Zhang, J. T. Popesku, C. J. Martyniuk, H. Xiong, P. Duarte-Guterman, L. Yao, X. Xia, and V. L. Trudeau Profiling neuroendocrine gene expression changes following fadrozole-induced estrogen decline in the female goldfish Physiol Genomics, August 7, 2009; 38(3): 351 - 361. [Abstract] [Full Text] [PDF]

				J. A. Mennigen, C. J. Martyniuk, K. Crump, H. Xiong, E. Zhao, J. Popesku, H. Anisman, A. R. Cossins, X. Xia, and V. L. Trudeau Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus) Physiol Genomics, November 12, 2008; 35(3): 273 - 282. [Abstract] [Full Text] [PDF]

				S. C. P. Renn, N. Aubin-Horth, and H. A. Hofmann Fish and chips: functional genomics of social plasticity in an African cichlid fish J. Exp. Biol., September 15, 2008; 211(18): 3041 - 3056. [Abstract] [Full Text] [PDF]