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This Article Full Text Full Text (PDF) Supplemental Figure and Table All Versions of this Article: 27/1/95    most recent 00039.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Seda, O. Articles by Kren, V. Search for Related Content PubMed PubMed Citation Articles by Seda, O. Articles by Kren, V.

Novel double-congenic strain reveals effects of spontaneously hypertensive rat chromosome 2 on specific lipoprotein subfractions and adiposity

Ondej eda ^1,2,3, Lucie edová ¹, Frantiek Lika ¹, Drahomíra Kenová ¹, Vratislav Prejzek ¹, Ludmila Kazdová ², Johanne Tremblay ³, Pavel Hamet ³ and Vladimír Ken ¹

¹ Institute of Biology and Medical Genetics of the First Faculty of Medicine of Charles University and the General Teaching Hospital, Prague, Czech Republic
² Department of Metabolism and Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
³ Centre de Recherche, Centre Hospitalier de l'Universite de Montreal, Montreal, Canada

We have developed a new, double-congenic rat strain BN-Lx.SHR2, which carries two distinct segments of chromosome 2 introgressed from the spontaneously hypertensive rat strain (SHR) into the genetic background of congenic strain BN-Lx, which was previously shown to express variety of metabolic syndrome features. In 16-wk-old male rats of BN-Lx and BN-Lx.SHR2 strains, we compared their glucose tolerance and triacylglycerol and cholesterol concentrations in 20 lipoprotein subfractions and the lipoprotein particle sizes under conditions of feeding standard and high-sucrose diets. Introgression of two distinct SHR-derived chromosome 2 segments resulted in decreased adiposity together with aggravation of glucose intolerance in the double-congenic strain. The BN-Lx.SHR2 rats were more sensitive to sucrose-induced rise in triacylglycerolemia. Although the total cholesterol concentrations of the two strains were comparable after the standard diet and even lower in BN-Lx.SHR2 after sucrose feeding, detailed analysis revealed that under both dietary conditions, the double-congenic strain had significantly higher cholesterol concentrations in low-density lipoprotein fractions and lower high-density lipoprotein fractions. We established a new inbred model showing dyslipidemia and mild glucose intolerance without obesity, attributable to specific genomic regions. For the first time, the chromosome 2 segments of SHR origin are shown to influence other than blood pressure-related features of metabolic syndrome or to be involved in relevant nutrigenomic interactions.

BN-Lx; triacylglycerol; cholesterol; metabolic syndrome; insulin resistance; obesity; comparative genomics