Physiol. Genomics Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 27: 65-78, 2006. First published July 5, 2006; doi:10.1152/physiolgenomics.00031.2006
1094-8341/06 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Table
Right arrow All Versions of this Article:
27/1/65    most recent
00031.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Radoja, N.
Right arrow Articles by Blumenberg, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radoja, N.
Right arrow Articles by Blumenberg, M.
Received 20 February 2006; accepted in final form 16 June 2006.
Physiological Genomics 27:65-78 (2006)
1094-8341/06 $8.00 © 2006 American Physiological Society

Transcriptional profiling of epidermal differentiation

Nada Radoja1, Alix Gazel1, Tomohiro Banno1,2, Shoichiro Yano1,3 and Miroslav Blumenberg1,4,5

1 Departments of Dermatology, New York University School of Medicine, New York, New York
4 Biochemistry, New York University School of Medicine, New York, New York
5 NYU Cancer Institute, New York University School of Medicine, New York, New York
2 Dermatology Department at the Institute of Clinical Medicine, Tsukuba University, Tsukuba, Ibaraki
3 Department of Dermatology, Faculty of Medicine, University of Tokyo, Tokyo, Japan

In epidermal differentiation basal keratinocytes detach from the basement membrane, stop proliferating, and express a new set of structural proteins and enzymes, which results in an impermeable protein/lipid barrier that protects us. To define the transcriptional changes essential for this process, we purified large quantities of basal and suprabasal cells from human epidermis, using the expression of ß4 integrin as the discriminating factor. The expected expression differences in cytoskeletal, cell cycle, and adhesion genes confirmed the effective separation of the cell populations. Using DNA microarray chips, we comprehensively identify the differences in genes expressed in basal and differentiating layers of the epidermis, including the ECM components produced by the basal cells, the proteases in both the basal and suprabasal cells, and the lipid and steroid metabolism enzymes in suprabasal cells responsible for the permeability barrier. We identified the signaling pathways specific for the two populations and found two previously unknown paracrine and one juxtacrine signaling pathway operating between the basal and suprabasal cells. Furthermore, using specific expression signatures, we identified a new set of late differentiation markers and mapped their chromosomal loci, as well as a new set of melanocyte-specific markers. The data represent a quantum jump in understanding the mechanisms of epidermal differentiation.

basal cells; epidermal differentiation complex; integrins; keratinization; melanocytes; microarrays






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.