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Physiol. Genomics 25: 525-527, 2006. First published February 28, 2006; doi:10.1152/physiolgenomics.00233.2005
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qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data

Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma

Quantitative real-time PCR is an important high-throughput method in the biomedical sciences. However, existing software has limitations in handling both relative and absolute quantification. We designed quantitative PCR data analysis and management system (qPCR-DAMS), a database tool based on Access 2003, to deal with such shortcomings by the addition of integrated mathematical procedures. qPCR-DAMS allows a user to choose among four methods for data processing within a single software package: 1) ratio relative quantification, 2) absolute level, 3) normalized absolute expression, and 4) ratio absolute quantification. qPCR-DAMS also provides a tool for multiple reference gene normalization. qPCR-DAMS has three quality control steps and a data display system to monitor data variation. In summary, qPCR-DAMS is a handy tool for real-time PCR users.

quantitative PCR data analysis and management system