Physiol. Genomics Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 25: 277-285, 2006. First published January 17, 2006; doi:10.1152/physiolgenomics.00302.2005
1094-8341/06 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
25/2/277    most recent
00302.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Castrop, H.
Right arrow Articles by Schnermann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castrop, H.
Right arrow Articles by Schnermann, J.
Received 7 December 2005; accepted in final form 10 January 2006.
Physiological Genomics 25:277-285 (2006)
1094-8341/06 $8.00 © 2006 American Physiological Society

Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene

H. Castrop 1, M. Oppermann 2, Y. Weiss 1, Y. Huang 2, D. Mizel 2, H. Lu 2, S. Germain 3, F. Schweda 1, F. Theilig 4, S. Bachmann 4, J. Briggs 2, A. Kurtz 1 and J. Schnermann 2

1 Institute of Physiology, University of Regensburg, Germany
2 National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland
3 Institut National de la Santé et de la Recherche Médicale (INSERM) Unit 36, College de France, Service d’Hematologie Biologique A, Hôpital European, Paris, France
4 Institute of Anatomy, Charité, Berlin, Germany

To assess the feasibility of using the renin promoter for expressing Cre recombinase in juxtaglomerular (JG) cells only, we generated five independent transgenic mouse lines (designated hRen-Cre) expressing Cre recombinase under control of a 12.2-kb human renin promoter. In the kidneys of adult mice Cre mRNA (RT-PCR) was found in the renal cortex, with Cre protein (immunohistochemistry) being localized in afferent arterioles and to a lower degree in interlobular arteries. Cre mRNA levels were regulated in a renin-typical fashion by changes in oral salt intake, water restriction, or isoproterenol infusion, indicating the presence of key regulatory elements within 12.2 kb of the 5'-flanking region of the human renin gene. hRen-Cre mice were interbred with both the ROSA26-EGFP and ROSA26-lacZ reporter strains to assess renin promoter activity from Cre-mediated excision of a floxed stop cassette and subsequent enhanced green fluorescent protein (EGFP) and ß-galactosidase (ß-gal) detection. In adult mice, ß-gal staining and EGFP were observed in afferent arterioles and interlobular arteries, overlapping with Cre protein expression. In addition, intense ß-gal staining was found in cortical and medullary collecting ducts where Cre expression was minimal. In embryonic kidneys, ß-gal staining was detected in the developing collecting duct system beginning at embryonic day 12, showing substantial activity of the human renin promoter in the branching ureteric bud. Our data indicate that besides its well-known activity in JG cells and renal vessels the human renin promoter is transiently active in the collecting duct system during kidney development, complicating the use of this approach for JG cell-specific excision of floxed targets.

renin; kidney development




This article has been cited by other articles:


Home page
 EndocrinologyHome page
V. T. Todorov, M. Desch, T. Schubert, and A. Kurtz
The Pal3 Promoter Sequence Is Critical for the Regulation of Human Renin Gene Transcription by Peroxisome Proliferator-Activated Receptor-{gamma}
Endocrinology, September 1, 2008; 149(9): 4647 - 4657.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
J.-M. Lalouel and A. Rohrwasser
Genetic Susceptibility to Essential Hypertension: Insight From Angiotensinogen
Hypertension, March 1, 2007; 49(3): 597 - 603.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Chen, S. M. Kim, M. Oppermann, R. Faulhaber-Walter, Y. Huang, D. Mizel, M. Chen, M. L. S. Lopez, L. S. Weinstein, R. A. Gomez, et al.
Regulation of renin in mice with Cre recombinase-mediated deletion of G protein Gs{alpha} in juxtaglomerular cells
Am J Physiol Renal Physiol, January 1, 2007; 292(1): F27 - F37.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhoux, D. R. Davis, and C. D. Sigmund
The Human Renin Kidney Enhancer Is Required to Maintain Base-line Renin Expression but Is Dispensable for Tissue-specific, Cell-specific, and Regulated Expression
J. Biol. Chem., November 17, 2006; 281(46): 35296 - 35304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.