HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 25/1/134    most recent 00262.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Liu, G. Articles by Johnson, E. A. Search for Related Content PubMed PubMed Citation Articles by Liu, G. Articles by Johnson, E. A.

Identification and function of hypoxia-response genes in Drosophila melanogaster

Institute of Molecular Biology, University of Oregon, Eugene, Oregon

Hypoxia, an insufficient level of oxygen in the cell, occurs during normal activity and also in pathological conditions such as ischemia and tumorigenesis. Although many hypoxia-response genes have been identified, an understanding of the functional role for these genes in the living animal is lacking. Here we present a genome-wide study of gene expression changes during hypoxia and then functionally test a subset of these genes for roles in survival and recovery from hypoxia. We found 79 genes with increased mRNA levels when adult flies were treated with 0.5% O₂ for 6 h. A subset of these genes had detectably increased levels in as short as 1 h of low-oxygen treatment. Mild hypoxia levels resulted in an increase in transcription levels for only 20 genes. Viability during hypoxia and recovery time from hypoxia-induced paralysis was examined in flies with a reduction in activity in hypoxia-response genes. The observed decreased viability and increased recovery time from paralysis in many of the lines demonstrate that the increased transcript levels seen after hypoxia are important for the response to low oxygen.

gene expression; microarray; low oxygen; paralysis; viability

This article has been cited by other articles:

				I. T. Helenius, T. Krupinski, D. W. Turnbull, Y. Gruenbaum, N. Silverman, E. A. Johnson, P. H. S. Sporn, J. I. Sznajder, and G. J. Beitel Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection PNAS, November 3, 2009; 106(44): 18710 - 18715. [Abstract] [Full Text] [PDF]

				H. Huang and G. G. Haddad Drosophila dMRP4 regulates responsiveness to O2 deprivation and development under hypoxia Physiol Genomics, May 11, 2007; 29(3): 260 - 266. [Abstract] [Full Text] [PDF]

				N. A. Baird, D. W. Turnbull, and E. A. Johnson Induction of the Heat Shock Pathway during Hypoxia Requires Regulation of Heat Shock Factor by Hypoxia-inducible Factor-1 J. Biol. Chem., December 15, 2006; 281(50): 38675 - 38681. [Abstract] [Full Text] [PDF]