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Physiol. Genomics 23: 206-216, 2005. First published August 16, 2005; doi:10.1152/physiolgenomics.00015.2005 Free Article
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Received 12 July 2005; accepted in final form 14 July 2005.
Physiological Genomics 23:206-216 (2005)
American Physiological Society © 2005 American Physiological Society

Article

Gene expression analysis suggests that EBF-1 and PPAR{gamma}2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics

Peter Åkerblad1, Robert Månsson3, Anna Lagergren3, Simonetta Westerlund1, Barbro Basta2, Ulrika Lind2, Anders Thelin2, Ramiro Gisler3, David Liberg3, Sven Nelander4, Krister Bamberg1 and Mikael Sigvardsson3

Departments of 1 Molecular Pharmacology and 2 Molecular Sciences, AstraZeneca Research and Development, Molndal; 3 Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund; and 4 Chalmers Technical University, Department of Mathematical Statistics, Goteborg, Sweden

ABSTRACT

Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR){gamma}2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR{gamma}2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR{gamma}2 induce adipocyte differentiation with comparable kinetics and efficiency.

adipogenesis; early B cell factor; peroxisome proliferator-activated receptor-{gamma}2




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