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Physiol. Genomics 23: 206-216, 2005. First published August 16, 2005; doi:10.1152/physiolgenomics.00015.2005
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Article

Gene expression analysis suggests that EBF-1 and PPAR2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics

Departments of ¹ Molecular Pharmacology and ² Molecular Sciences, AstraZeneca Research and Development, Molndal; ³ Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund; and ⁴ Chalmers Technical University, Department of Mathematical Statistics, Goteborg, Sweden

ABSTRACT

Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR2 induce adipocyte differentiation with comparable kinetics and efficiency.

adipogenesis; early B cell factor; peroxisome proliferator-activated receptor-2

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