| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin
The understanding of common and tissue-specific molecular alterations in diabetes, particularly at early stages, is limited and fragmental. In the present study, we systematically compared transcriptome responses in four important diabetic target tissues in rats with 2 wk of streptozotocin (STZ)-induced diabetes. At this stage of diabetes, the skeletal muscle exhibited the highest transcriptome sensitivity to the STZ treatment with nearly 17% of the transcriptome being altered (false discovery rate, 1.6%) compared with 
3% in the cardiac left ventricle, renal cortex, and retina. Similarity in transcriptome response among tissues was low, with the highest similarity being 2.2% between skeletal muscle and the left ventricle. Several biological processes or cellular components, such as lipid metabolism in the left ventricle and collagen in the renal cortex, were significantly overrepresented in the responsive genes than in the entire array. Particularly interesting cases of common or tissue-specific regulation included decorin and CD36, which were upregulated in several tissues, and serum/glucocorticoid-regulated kinase and four and a half LIM domains 2, which were upregulated only in the renal cortex. Further biochemical analyses indicated that the thiol and oxidative stress pathway was altered in a tissue-specific manner at several levels including transcript abundance, content of reduced thiols, and lipid peroxidation, providing an example of the potential biological relevance of tissue-specific transcript regulation. These results provided a transcriptome-wide view of the molecular alterations across several key tissues in early diabetes. It appears that both common pathways and, perhaps more importantly, tissue-specific mechanisms are involved in the adaptation to diabetes or the initiation of diabetic complications.
diabetic complications; microarray; gene expression; decorin; CD36
This article has been cited by other articles:
|
|
 |
|
  M. Liang, N. H. Lee, H. Wang, A. S. Greene, A. E. Kwitek, M. L. Kaldunski, T. V. Luu, B. C. Frank, S. Bugenhagen, H. J. Jacob, et al. Molecular networks in Dahl salt-sensitive hypertension based on transcriptome analysis of a panel of consomic rats Physiol Genomics, June 10, 2008; 34(1): 54 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Tian, A. S. Greene, K. Usa, I. R. Matus, J. Bauwens, J. L. Pietrusz, A. W. Cowley Jr, and M. Liang Renal Regional Proteomes in Young Dahl Salt-Sensitive Rats Hypertension, April 1, 2008; 51(4): 899 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Tian, A. S. Greene, J. L. Pietrusz, I. R. Matus, and M. Liang MicroRNA-target pairs in the rat kidney identified by microRNA microarray, proteomic, and bioinformatic analysis Genome Res., March 1, 2008; 18(3): 404 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, F. Park, J. L. Pietrusz, G. Jia, R. J. Singh, B. C. Netzel, and M. Liang Suppression of 11{beta}-hydroxysteroid dehydrogenase type 1 with RNA interference substantially attenuates 3T3-L1 adipogenesis Physiol Genomics, February 19, 2008; 32(3): 343 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. van Lunteren and M. Moyer Oxidoreductase, morphogenesis, extracellular matrix, and calcium ion-binding gene expression in streptozotocin-induced diabetic rat heart Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E759 - E768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Usa, R. J. Singh, B. C. Netzel, Y. Liu, H. Raff, and M. Liang Renal interstitial corticosterone and 11-dehydrocorticosterone in conscious rats Am J Physiol Renal Physiol, July 1, 2007; 293(1): F186 - F192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Glyn-Jones, S. Song, M. A. Black, A. R. J. Phillips, S. Y. Choong, and G. J. S. Cooper Transcriptomic analysis of the cardiac left ventricle in a rodent model of diabetic cardiomyopathy: molecular snapshot of a severe myocardial disease Physiol Genomics, February 12, 2007; 28(3): 284 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Liang and J. L. Pietrusz Thiol-Related Genes in Diabetic Complications: A Novel Protective Role for Endogenous Thioredoxin 2 Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 77 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Lehti, M. Silvennoinen, R. Kivela, H. Kainulainen, and J. Komulainen Effects of streptozotocin-induced diabetes and physical training on gene expression of extracellular matrix proteins in mouse skeletal muscle Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E900 - E907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang and B. Ventura Physiological genomics in PG and beyond: October to December 2005 Physiol Genomics, December 14, 2005; 24(1): 1 - 3. [Full Text] [PDF]
|
|
|
|
|
|
M. Liang and B. Ventura Physiological genomics in PG and beyond: July to September 2005 Physiol Genomics, October 17, 2005; 23(2): 119 - 124. [Full Text] [PDF]
|
|
|
|
|
|
C. J. Sullivan, T. H. Teal, I. P. Luttrell, K. B. Tran, M. A. Peters, and H. Wessells Microarray analysis reveals novel gene expression changes associated with erectile dysfunction in diabetic rats Physiol Genomics, October 17, 2005; 23(2): 192 - 205. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
