Physiol. Genomics Genetics/Genomics of Vascular Disease Workshop
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 21: 59-69, 2005. First published December 28, 2004; doi:10.1152/physiolgenomics.00257.2004 Free Article
1094-8341/05 $8.00
This Article
Free upon publication Free Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrowFree Article All Versions of this Article:
21/1/59    most recent
00257.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diez-Tascón, C.
Right arrow Articles by Crawford, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Diez-Tascón, C.
Right arrow Articles by Crawford, A. M.
Received 29 October 2004; accepted in final form 22 December 2004.
Physiological Genomics 21:59-69 (2005)
1094-8341/05 $8.00 © 2005 American Physiological Society

Microarray analysis of selection lines from outbred populations to identify genes involved with nematode parasite resistance in sheep

Cristina Diez-Tascón1, Orla M. Keane1, Theresa Wilson1, Amonida Zadissa1, Dianne L. Hyndman2, David B. Baird3, John C. McEwan2 and Allan M. Crawford2

1 AgResearch, Molecular Biology Unit, Department of Biochemistry, University of Otago, Dunedin
2 AgResearch, Invermay Agricultural Centre, Mosgiel
3 AgResearch, Lincoln, New Zealand

Gastrointestinal nematodes infect sheep grazing contaminated pastures. Traditionally, these have been controlled with anthelmintic drenching. The selection of animals resistant to nematodes is an alternative to complete reliance on drugs, but the genetic basis of host resistance is poorly understood. Using a 10,204 bovine cDNA microarray, we have examined differences in gene expression between genetically resistant and susceptible lambs previously field challenged with larval nematodes. Northern blot analysis for a selection of genes validated the data obtained from the microarrays. The results identified over one hundred genes that were differentially expressed based on conservative criteria. The microarray results were further analyzed to identify promoter motifs common to the differentially expressed genes. Motifs identified in upregulated gene promoters were primarily restricted to those promoters; however, motifs identified in downregulated gene promoters were also found in the promoters of upregulated genes but not in the promoters of genes whose expression was unaltered. Protein Annotators’ Assistant was used for lexical analysis of the differentially expressed genes, and Gene Ontology was used to look for metabolic and cell signaling pathways associated with parasite resistance. Two pathways represented by genes differentially expressed in resistant animals were those involved with the development of an acquired immune response and those related to the structure of the intestine smooth muscle. Genes involved in these processes appear from our analysis to be key genetic determinants of parasite resistance.

gastrointestinal nematodes; major histocompatibility complex class II; transgelin; actin




This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
O. M. Keane, K. G. Dodds, A. M. Crawford, and J. C. McEwan
Transcriptional profiling of Ovis aries identifies Ovar-DQA1 allele frequency differences between nematode-resistant and susceptible selection lines
Physiol Genomics, August 20, 2007; 30(3): 253 - 261.
[Abstract] [Full Text] [PDF]

Home page
 Proc R Soc BHome page
M.R Hutchings, K.J Knowler, R McAnulty, and J.C McEwan
Genetically resistant sheep avoid parasites to a greater extent than do susceptible sheep
Proc R Soc B, August 7, 2007; 274(1620): 1839 - 1844.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
A. Oshlack, A. E. Chabot, G. K. Smyth, and Y. Gilad
Using DNA microarrays to study gene expression in closely related species
Bioinformatics, May 15, 2007; 23(10): 1235 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. Avivi, L. Brodsky, E. Nevo, and M. R. Band
Differential expression profiling of the blind subterranean mole rat Spalax ehrenbergi superspecies: bioprospecting for hypoxia tolerance
Physiol Genomics, January 12, 2007; 27(1): 54 - 64.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
J. Somers, C. Smith, M. Donnison, D. N Wells, H. Henderson, L. McLeay, and P L Pfeffer
Gene expression profiling of individual bovine nuclear transfer blastocysts.
Reproduction, June 1, 2006; 131(6): 1073 - 1084.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.