Physiol. Genomics Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 20: 211-223, 2005. First published December 7, 2004; doi:10.1152/physiolgenomics.00229.2003
1094-8341/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
20/3/211    most recent
00229.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iacobas, D. A.
Right arrow Articles by Spray, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iacobas, D. A.
Right arrow Articles by Spray, D. C.
Received 31 December 2003; accepted in final form 30 November 2004.
Physiological Genomics 20:211-223 (2005)
1094-8341/05 $5.00 © 2005 American Physiological Society

Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart

Dumitru A. Iacobas 1, Sanda Iacobas 1, W. E. I. Li 1, Georg Zoidl 3, Rolf Dermietzel 3 and David C. Spray 1,2

1 Department of Neuroscience Albert Einstein College of Medicine, Bronx, New York
2 Department of Cardiology, Albert Einstein College of Medicine, Bronx, New York
3 Department of Neuroanatomy and Molecular Brain Research, Ruhr-University, Bochum, Germany

We have used mouse 27k cDNA arrays to compare gene expression patterns in four sets of three hearts each of neonatal wild types and four sets of three hearts each of littermates lacking the major cardiac gap junction protein, connexin43 (Cx43). Each individual set of hearts was hybridized against aliquots of an RNA standard prepared from selected mouse tissues, allowing calculation of variability and coordination of gene expression among the samples from both genotypes. Overall variance of gene expression was found to be markedly higher in wild-type hearts than in those from Cx43 null littermates. Expression levels of 586 of 5,613 adequately quantifiable distinct genes with known protein products were statistically altered in the Cx43 null hearts, 38 upregulated and 548 downregulated compared with wild types. Downregulation was confirmed for seven tested genes by quantitative RT-PCR. Functions of proteins encoded by the altered genes encompassed all functional categories, with largest percent changes in genes involved in intracellular transport and transcription factors. Among the downregulated genes in the Cx43 null hearts were those related to neuronal and glial function, suggesting that cardiac innervation might be compromised as a consequence of Cx43 deletion. This was supported by immunodetection of sympathetic innervation, using antibodies to the synaptic vesicle protein synaptophysin and to the adrenergic nerve terminal marker tyrosine hydroxylase. These findings reinforce the proposal that the cardiac abnormality in Cx43 null animals may be contributed by altered innervation and indicate that Cx43 deletion has consequences in addition to reduced intercellular communication.

cDNA array; expression coordination; cardiac innervation; transcription control




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W.-C. Sin, M. Tse, N. Planque, B. Perbal, P. D. Lampe, and C. C. Naus
Matricellular Protein CCN3 (NOV) Regulates Actin Cytoskeleton Reorganization
J. Biol. Chem., October 23, 2009; 284(43): 29935 - 29944.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. B. Danik, G. Rosner, J. Lader, D. E. Gutstein, G. I. Fishman, and G. E. Morley
Electrical remodeling contributes to complex tachyarrhythmias in connexin43-deficient mouse hearts
FASEB J, April 1, 2008; 22(4): 1204 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
N. Fitzner, S. Clauberg, F. Essmann, J. Liebmann, and V. Kolb-Bachofen
Human Skin Endothelial Cells Can Express All 10 TLR Genes and Respond to Respective Ligands
Clin. Vaccine Immunol., January 1, 2008; 15(1): 138 - 146.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. J. Crocker, J. K. Whitmire, R. F. Frausto, P. Chertboonmuang, P. D. Soloway, J. L. Whitton, and I. L. Campbell
Persistent Macrophage/Microglial Activation and Myelin Disruption after Experimental Autoimmune Encephalomyelitis in Tissue Inhibitor of Metalloproteinase-1-Deficient Mice
Am. J. Pathol., December 1, 2006; 169(6): 2104 - 2116.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
C. Fan, D. A. Iacobas, D. Zhou, Q. Chen, J. K. Lai, O. Gavrialov, and G. G. Haddad
Gene expression and phenotypic characterization of mouse heart after chronic constant or intermittent hypoxia
Physiol Genomics, August 11, 2005; 22(3): 292 - 307.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.