HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Yao, J. Articles by Smith, G. W. Search for Related Content PubMed PubMed Citation Articles by Yao, J. Articles by Smith, G. W.

Generation of a bovine oocyte cDNA library and microarray: resources for identification of genes important for follicular development and early embryogenesis

¹ Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, West Virginia 26506
² United States Department of Agriculture, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, Nebraska 68933
³ Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, Michigan 48824
⁴ Department of Animal Science, Michigan State University, East Lansing, Michigan 48824
⁵ Department of Physiology, Michigan State University, East Lansing, Michigan 48824
⁶ Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan 48824

The oocyte is a key regulator of ovarian folliculogenesis and early embryonic development. However, the composition of the oocyte transcriptome and identities and functions of key oocyte-specific genes involved in the above processes are relatively unknown. Using a PCR-based cDNA amplification method (SMART technology), we constructed a bovine oocyte cDNA library. Analysis of 230 expressed sequence tags (ESTs) from this library identified 102 unique sequences. Although some correspond to housekeeping genes (e.g., ribosomal protein L15) and some represent genes previously known to be expressed in oocytes and other tissues, most encode for genes whose expression in mammalian oocytes has not been reported previously (e.g., cocaine- and amphetamine-regulated transcript) or genes of unknown function. Sixteen did not show significant sequence similarity to any entries in the GenBank database and were classified as novel. Using over 2,000 unsequenced, randomly selected cDNA clones from the library, we constructed an oocyte microarray and performed experiments to identify genes preferentially expressed in fetal ovary (an enriched source of oocytes) relative to somatic tissues. Eleven clones were identified by microarray analysis with consistently higher expression in fetal ovaries (collected from animals at days 210–260 of gestation) compared with spleen and liver. DNA sequence analysis of these clones revealed that two correspond to JY-1, a novel bovine oocyte-specific gene. The remaining nine clones represent five identified genes and one additional completely novel gene. Increased abundance of mRNA in fetal ovary for five of the six genes identified was confirmed by real-time PCR. Results demonstrate the potential utility of these unique resources for identification of oocyte-expressed genes potentially important for reproductive function.

cattle; egg; expressed sequence tag; ovary

This article has been cited by other articles:

				M. Vallee, K. Aiba, Y. Piao, M.-F. Palin, M. S H Ko, and M.-A. Sirard Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species Reproduction, April 1, 2008; 135(4): 439 - 448. [Abstract] [Full Text] [PDF]

				A Bonnet, R Dalbies-Tran, and M A Sirard Opportunities and challenges in applying genomics to the study of oogenesis and folliculogenesis in farm animals Reproduction, February 1, 2008; 135(2): 119 - 128. [Abstract] [Full Text] [PDF]

				S L Rodriguez-Zas, K Schellander, and H A Lewin Biological interpretations of transcriptomic profiles in mammalian oocytes and embryos Reproduction, February 1, 2008; 135(2): 129 - 139. [Abstract] [Full Text] [PDF]

				A. Bettegowda, J. Yao, A. Sen, Q. Li, K.-B. Lee, Y. Kobayashi, O. V. Patel, P. M. Coussens, J. J. Ireland, and G. W. Smith JY-1, an oocyte-specific gene, regulates granulosa cell function and early embryonic development in cattle PNAS, November 6, 2007; 104(45): 17602 - 17607. [Abstract] [Full Text] [PDF]

				M. Salem, P. B. Kenney, C. E. Rexroad 3rd, and J. Yao Microarray gene expression analysis in atrophying rainbow trout muscle: a unique nonmammalian muscle degradation model Physiol Genomics, December 13, 2006; 28(1): 33 - 45. [Abstract] [Full Text] [PDF]

				H.-J. Yu, P. Hogan, and V. Sundaresan Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling Plant Physiology, December 1, 2005; 139(4): 1853 - 1869. [Abstract] [Full Text] [PDF]

				S. Pennetier, S. Uzbekova, C. Guyader-Joly, P. Humblot, P. Mermillod, and R. Dalbies-Tran Genes Preferentially Expressed in Bovine Oocytes Revealed by Subtractive and Suppressive Hybridization Biol Reprod, October 1, 2005; 73(4): 713 - 720. [Abstract] [Full Text] [PDF]