| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Toolbox
Cardiovascular Research Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215
Mouse models mimicking human diseases are important tools in trying to understand the underlying mechanisms of many disease states. Several surgical models have been described that mimic human myocardial infarction (MI) and pressure-overload-induced cardiac hypertrophy. However, there are very few detailed descriptions for performing these surgical techniques in mice. Consequently, the number of laboratories that are proficient in performing cardiac surgical procedures in mice has been limited. Microarray technologies measure the expression of thousands of genes simultaneously, allowing for the identification of genes and pathways that may potentially be involved in the disease process. The statistical analysis of microarray experiments is highly influenced by the amount of variability in the experiment. To keep the number of required independent biological replicates and the associated costs of the study to a minimum, it is critical to minimize experimental variability by optimizing the surgical procedures. The aim of this publication was to provide a detailed description of techniques required to perform mouse cardiac surgery, such that these models can be utilized for genomic studies. A description of three major surgical procedures has been provided: 1) aortic constriction, 2) pulmonary artery banding, 3) MI (including ischemia-reperfusion). Emphasis has been placed on technical procedures with the inclusion of thorough descriptions of all equipment and devices employed in surgery, as well as the application of such techniques for expression profiling studies. The cardiac surgical techniques described have been, and will continue to be, important for elucidating the molecular mechanisms of cardiac hypertrophy and failure with high-throughput technology.
cardiac hypertrophy; heart failure; surgical procedures; gene expression profile
This article has been cited by other articles:
|
|
 |
|
  J. L. Wright, M. Cosio, and A. Churg Animal models of chronic obstructive pulmonary disease Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L1 - L15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Duka, E. Kintsurashvili, I. Duka, D. Ona, T. A. Hopkins, M. Bader, I. Gavras, and H. Gavras Angiotensin-Converting Enzyme Inhibition After Experimental Myocardial Infarct: Role of the Kinin B1 and B2 Receptors Hypertension, May 1, 2008; 51(5): 1352 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Jiang, S. R. Hall, M. P.W. Moos, R. Y. Cao, S. Ishii, K. O. Ogunyankin, L. G. Melo, and C. D. Funk Endothelial Cysteinyl Leukotriene 2 Receptor Expression Mediates Myocardial Ischemia-Reperfusion Injury Am. J. Pathol., March 1, 2008; 172(3): 592 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Shioura, D. L. Geenen, and P. H. Goldspink Assessment of cardiac function with the pressure-volume conductance system following myocardial infarction in mice Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2870 - H2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Barrick, M. Rojas, R. Schoonhoven, S. S. Smyth, and D. W. Threadgill Cardiac response to pressure overload in 129S1/SvImJ and C57BL/6J mice: temporal- and background-dependent development of concentric left ventricular hypertrophy Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2119 - H2130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bourdel, S. Matsuzaki, J.-E. Bazin, J.-L. Pouly, G. Mage, and M. Canis Peritoneal tissue-oxygen tension during a carbon dioxide pneumoperitoneum in a mouse laparoscopic model with controlled respiratory support Hum. Reprod., April 1, 2007; 22(4): 1149 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. McMullen, F. Amirahmadi, E. A. Woodcock, M. Schinke-Braun, R. D. Bouwman, K. A. Hewitt, J. P. Mollica, L. Zhang, Y. Zhang, T. Shioi, et al. Protective effects of exercise and phosphoinositide 3-kinase(p110{alpha}) signaling in dilated and hypertrophic cardiomyopathy PNAS, January 9, 2007; 104(2): 612 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xu, N. Li, Y. He, V. Timofeyev, L. Lu, H.-J. Tsai, I.-H. Kim, D. Tuteja, R. K. P. Mateo, A. Singapuri, et al. Prevention and reversal of cardiac hypertrophy by soluble epoxide hydrolase inhibitors PNAS, December 5, 2006; 103(49): 18733 - 18738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P VISCONTI and R. R MARKWALD Recruitment of New Cells into the Postnatal Heart: Potential Modification of Phenotype by Periostin Ann. N.Y. Acad. Sci., October 1, 2006; 1080(1): 19 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bisping, S. Ikeda, S. W. Kong, O. Tarnavski, N. Bodyak, J. R. McMullen, S. Rajagopal, J. K. Son, Q. Ma, Z. Springer, et al. Gata4 is required for maintenance of postnatal cardiac function and protection from pressure overload-induced heart failure PNAS, September 26, 2006; 103(39): 14471 - 14476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Mollmann, H. M. Nef, S. Kostin, C. von Kalle, I. Pilz, M. Weber, J. Schaper, C. W. Hamm, and A. Elsasser Bone marrow-derived cells contribute to infarct remodelling Cardiovasc Res, September 1, 2006; 71(4): 661 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Guerra, R. Roncon-Albuquerque Jr, A. P. Lourenco, I. Falcao-Pires, P. Cibrao-Coutinho, and A. F. Leite-Moreira Remote myocardium gene expression after 30 and 120 min of ischaemia in the rat Exp Physiol, March 1, 2006; 91(2): 473 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Liang and B. Ventura Physiological genomics in PG and beyond: October to December 2005 Physiol Genomics, December 14, 2005; 24(1): 1 - 3. [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Saraiva, K. M. Minhas, S. V.Y. Raju, L. A. Barouch, E. Pitz, K. H. Schuleri, K. Vandegaer, D. Li, and J. M. Hare Deficiency of Neuronal Nitric Oxide Synthase Increases Mortality and Cardiac Remodeling After Myocardial Infarction: Role of Nitroso-Redox Equilibrium Circulation, November 29, 2005; 112(22): 3415 - 3422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. L. Tang, K. Qian, Y. C. Zhang, L. Shen, and M. I. Phillips A Vigilant, Hypoxia-Regulated Heme Oxygenase-1 Gene Vector in the Heart Limits Cardiac Injury After Ischemia-Reperfusion In Vivo Journal of Cardiovascular Pharmacology and Therapeutics, October 1, 2005; 10(4): 251 - 263. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Ricci, U. Eriksson, G. Y. Oudit, R. Eferl, A. Akhmedov, I. Sumara, G. Sumara, Z. Kassiri, J.-P. David, L. Bakiri, et al. Distinct functions of junD in cardiac hypertrophy and heart failure Genes & Dev., January 15, 2005; 19(2): 208 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al. Functional Genomics of Lung Disease Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81. [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. McMullen, T. Shioi, L. Zhang, O. Tarnavski, M. C. Sherwood, A. L. Dorfman, S. Longnus, M. Pende, K. A. Martin, J. Blenis, et al. Deletion of Ribosomal S6 Kinases Does Not Attenuate Pathological, Physiological, or Insulin-Like Growth Factor 1 Receptor-Phosphoinositide 3-Kinase-Induced Cardiac Hypertrophy Mol. Cell. Biol., July 15, 2004; 24(14): 6231 - 6240. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
