HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 16: 240-246, 2004. First published November 18, 2003; doi:10.1152/physiolgenomics.00131.2003
1094-8341/04 $5.00

This Article Free upon publication Full Text Full Text (PDF) All Versions of this Article: 16/2/240    most recent 00131.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Lavoie, J. L. Articles by Sigmund, C. D. Search for Related Content PubMed PubMed Citation Articles by Lavoie, J. L. Articles by Sigmund, C. D.

Localization of renin expressing cells in the brain, by use of a REN-eGFP transgenic model

¹ Departments of Internal Medicine and Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242
² Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242
³ Roswell Park Cancer Institute, Buffalo, New York 14263

Immunoreactive renin has been reported in the hypothalamus and cerebellar cortex in the rodent brain and in neurons in all areas of the human brain. Despite these observations and the clear documentation of the expression of the other renin-angiotensin system genes in the brain, the notion that renin is endogenously expressed in the brain remains very controversial and undefined. This controversy no doubt arises because the level of renin expression in the brain is below the detection threshold of most standard assays. A transgenic mouse expressing enhanced green fluorescence protein (eGFP) under the control of the mouse renin promoter was recently reported. This model expresses eGFP in the kidney, which responds appropriately to both developmental and physiological stimuli. We therefore used eGFP as a sensitive marker to identify renin-expressing cells in the brain. We identified eGFP-containing cells in specific areas of the brain, including cerebellum, hippocampus, dorsal motor nucleus of the vagus, inferior olivary nucleus, reticular formation, rostral ventrolateral medulla, central nucleus of the amygdala, lateral parabrachial nucleus, mesencephalic trigeminal nucleus, bed nucleus of stria terminalis, and subfornical organ. By colabeling with neuron- or glia (astrocytes or oligodendrocytes)-specific antisera, we have determined the eGFP-positive cells to be mainly neuronal. These findings therefore strongly support the primary expression of renin mRNA in the brain in regions controlling cardiovascular function.

ACE/angiotensin receptors, gene expression, genetically altered mice; renin; brain; neuron; astrocyte; oligodendrocytes

This article has been cited by other articles:

				J. L. Grobe, D. Xu, and C. D. Sigmund An Intracellular Renin-Angiotensin System in Neurons: Fact, Hypothesis, or Fantasy Physiology, August 1, 2008; 23(4): 187 - 193. [Abstract] [Full Text] [PDF]

				Z. Lin, Y. Chen, W. Zhang, A. F. Chen, S. Lin, and M. Morris RNA interference shows interactions between mouse brainstem angiotensin AT1 receptors and angiotensin-converting enzyme 2 Exp Physiol, May 1, 2008; 93(5): 676 - 684. [Abstract] [Full Text] [PDF]

				I. H. Zucker Angiotensin-Converting Enzyme 2: A New Player in Central Sympathetic Regulation? Circ. Res., March 28, 2008; 102(6): 628 - 629. [Full Text] [PDF]

				Y. Feng, X. Yue, H. Xia, S. M. Bindom, P. J. Hickman, C. M. Filipeanu, G. Wu, and E. Lazartigues Angiotensin-Converting Enzyme 2 Overexpression in the Subfornical Organ Prevents the Angiotensin II-Mediated Pressor and Drinking Responses and Is Associated With Angiotensin II Type 1 Receptor Downregulation Circ. Res., March 28, 2008; 102(6): 729 - 736. [Abstract] [Full Text] [PDF]

				J. L. Lavoie, X. Liu, R. A. Bianco, T. G. Beltz, A. K. Johnson, and C. D. Sigmund Evidence Supporting a Functional Role for Intracellular Renin in the Brain Hypertension, March 1, 2006; 47(3): 461 - 466. [Abstract] [Full Text] [PDF]

				M. Sherrod, D. R. Davis, X. Zhou, M. D. Cassell, and C. D. Sigmund Glial-specific ablation of angiotensinogen lowers arterial pressure in renin and angiotensinogen transgenic mice Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1763 - R1769. [Abstract] [Full Text] [PDF]

				M. Sherrod, X. Liu, X. Zhang, and C. D. Sigmund Nuclear localization of angiotensinogen in astrocytes Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R539 - R546. [Abstract] [Full Text] [PDF]

				J. L. Lavoie, M. D. Cassell, K. W. Gross, and C. D. Sigmund Adjacent Expression of Renin and Angiotensinogen in the Rostral Ventrolateral Medulla Using a Dual-Reporter Transgenic Model Hypertension, May 1, 2004; 43(5): 1116 - 1119. [Abstract] [Full Text] [PDF]