HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 16/1/153    most recent 00099.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Nobis, W. Articles by Coussens, P. M. Search for Related Content PubMed PubMed Citation Articles by Nobis, W. Articles by Coussens, P. M.

Toolbox

Development of a porcine brain cDNA library, EST database, and microarray resource

¹ Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan 48824
³ Animal Behavior and Welfare Group, Department of Animal Science, Michigan State University, East Lansing, Michigan 48824
² Texas Instruments, Stafford, Texas 77477

Recent developments in expressed sequence tag (EST) and cDNA microarray technology have had a dramatic impact on the ability of scientists to study responses of thousands of genes to internal and external stimuli. In neurobiology, studies of the human brain have been expanding rapidly by use of functional genomics techniques. To enhance these studies and allow use of a porcine brain model, a normalized porcine brain cDNA library (PBL) has been generated and used as a base for EST discovery and microarray generation. In this report, we discuss initial sequence analysis of 965 clones from this resource. Our data revealed that library normalization successfully reduced the number of clones representing highly abundant cDNA species and overall clone redundancy. Cluster analysis revealed over 800 unique cDNA species representing a redundancy rate for the normalized library of 6.9% compared with 29.4% before normalization. Sequence information, BLAST results, and TIGR cluster matches for these ESTs are publicly available via a web-accessible database (http://nbfgc.msu.edu). A cDNA microarray was created using 877 unique porcine brain EST amplicons spotted in triplicate on glass slides. This microarray was assessed by performing a series of experiments designed to test hybridization efficiency and false-positive rate. Our results indicate that the PBL cDNA microarray is a robust tool for studies of brain gene expression using swine as a model system.

pig; behavior; swine; CNS; model system

This article has been cited by other articles:

				R. Davoli and S. Braglia Molecular approaches in pig breeding to improve meat quality Brief Funct Genomic Proteomic, January 21, 2008; (2008) elm036v1. [Abstract] [Full Text] [PDF]

				A. Reverter, N. J. Hudson, Y. Wang, S.-H. Tan, W. Barris, K. A. Byrne, S. M. McWilliam, C. D. K. Bottema, A. Kister, P. L. Greenwood, et al. A gene coexpression network for bovine skeletal muscle inferred from microarray data Physiol Genomics, December 13, 2006; 28(1): 76 - 83. [Abstract] [Full Text] [PDF]

				M. Liang and B. Ventura Physiological genomics in PG and beyond: October to December 2005 Physiol Genomics, December 14, 2005; 24(1): 1 - 3. [Full Text] [PDF]

				P. R. Nambiar, S. R. Boutin, R. Raja, and D. W. Rosenberg Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia Vet. Pathol., November 1, 2005; 42(6): 735 - 752. [Abstract] [Full Text] [PDF]