HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 15: 199-208, 2003. First published September 9, 2003; doi:10.1152/physiolgenomics.00086.2003
1094-8341/03 $5.00

This Article Free upon publication Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 15/3/199    most recent 00086.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Matsui, Y. Articles by Makuuchi, M. Search for Related Content PubMed PubMed Citation Articles by Matsui, Y. Articles by Makuuchi, M.

Identification of gene expression profile in tolerizing murine cardiac allograft by costimulatory blockade

¹ Departments of Hepato-Biliary-Pancreatic and Transplantation Surgery
² Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655
³ Department of Immunology, Juntendo University School of Medicine, Tokyo 113-8421
⁴ Biostatistics/ Epidemiology and Preventive Health Sciences, School of Health Sciences and Nursing, University of Tokyo, Tokyo 113-8655
⁵ Genome Sciences, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan
⁶ Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan

The induction of specific tolerance would be the ultimate achievement in transplant immunology, but the precise mechanisms of immunologic tolerance remain largely unknown. Here, we investigated global gene expression analysis in tolerizing murine cardiac allografts by means of oligonucleotide microarrays. Tolerance induction was achieved in cardiac allografts from BALB/c to C57BL/6 mice by daily intraperitoneal injection of anti-CD80 and anti-CD86 monoclonal antibodies (mAbs). Comparative analysis revealed 64 genes to be induced more extensively in the tolerizing than in the syngeneic isografts, and 16 genes than in the rejecting allografts. Two genes were specifically upregulated in the tolerizing allografts. In the tolerizing allografts there were induced marked expressions of a number of genes for pro-inflammatory factors, including interferon--inducible cytokines and chemokines, as well as apoptosis-related genes, which were also upregulated in the rejecting allografts. Moreover, these gene expression patterns continued to be upregulated more than 70 days posttransplant. These results provide evidence that immunologic tolerance can be induced and maintained in the presence of prominent pro-inflammatory gene expression in vivo.

transplantation; immunologic tolerance; interferon-; chemokine; DNA microarray

This article has been cited by other articles:

				J. D. Lande, J. Patil, N. Li, T. R. Berryman, R. A. King, and M. I. Hertz Novel Insights into Lung Transplant Rejection by Microarray Analysis Proceedings of the ATS, January 1, 2007; 4(1): 44 - 51. [Abstract] [Full Text] [PDF]

				M. Liang and B. Ventura Physiological genomics in PG and beyond: July to September 2005 Physiol Genomics, October 17, 2005; 23(2): 119 - 124. [Full Text] [PDF]