| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, M5G 1L5, Canada
2 Cardiovascular Genome Unit, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115
Understanding how vertebrates respond to hypoxia can have important clinical implications. Fish have evolved the ability to survive long exposure to low oxygen levels. However, little is known about the specific changes in gene expression that result from hypoxia. In this study we used a zebrafish cDNA microarray to examine the expression of >4,500 genes in zebrafish embryos exposed to 24 h of hypoxia during development. We tested the hypotheses that hypoxia changes gene expression profile of the zebrafish embryos and that these changes can be reverted by reexposure to a normoxic (20.8% O2) environment. Our data were consistent with both of these hypotheses: indicating that zebrafish embryos undergo adaptive changes in gene expression in response to hypoxia. Our study provides a striking genetic portrait of the zebrafish embryos’ adaptive responses to hypoxic stress and demonstrates the utility of the microarray technology as a tool for analyzing complex developmental processes in the zebrafish.
Danio rerio; cDNA microarray
This article has been cited by other articles:
|
|
 |
|
  A. J. Esbaugh, S. F. Perry, and K. M. Gilmour Hypoxia-inducible carbonic anhydrase IX expression is insufficient to alleviate intracellular metabolic acidosis in the muscle of zebrafish, Danio rerio Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2009; 296(1): R150 - R160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-Y. Chou, C.-D. Hsiao, S.-C. Chen, I-W. Chen, S.-T. Liu, and P.-P. Hwang Effects of hypothermia on gene expression in zebrafish gills: upregulation in differentiation and function of ionocytes as compensatory responses J. Exp. Biol., October 1, 2008; 211(19): 3077 - 3084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Brown-Peterson, C. S. Manning, V. Patel, N. D. Denslow, and M. Brouwer Effects of Cyclic Hypoxia on Gene Expression and Reproduction in a Grass Shrimp, Palaemonetes pugio Biol. Bull., February 1, 2008; 214(1): 6 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Baden, J. Murray, R. A. Capaldi, and K. Guillemin Early Developmental Pathology Due to Cytochrome c Oxidase Deficiency Is Revealed by a New Zebrafish Model J. Biol. Chem., November 30, 2007; 282(48): 34839 - 34849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gray, I. M. Packham, F. Wurmser, N. C. Eastley, P. G. Hellewell, P. W. Ingham, D. C. Crossman, and T. J.A. Chico Ischemia Is Not Required for Arteriogenesis in Zebrafish Embryos Arterioscler. Thromb. Vasc. Biol., October 1, 2007; 27(10): 2135 - 2141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. de la Vega, M. R. Hall, K. J. Wilson, A. Reverter, R. G. Woods, and B. M. Degnan Stress-induced gene expression profiling in the black tiger shrimp Penaeus monodon Physiol Genomics, September 11, 2007; 31(1): 126 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Chen, S.-J. Wang, J.-Z. Du, and X.-C. Chen Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R516 - R526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. McClelland, P. M. Craig, K. Dhekney, and S. Dipardo Temperature- and exercise-induced gene expression and metabolic enzyme changes in skeletal muscle of adult zebrafish (Danio rerio) J. Physiol., December 1, 2006; 577(2): 739 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Martyniuk, H. Xiong, K. Crump, S. Chiu, R. Sardana, A. Nadler, E. R. Gerrie, X. Xia, and V. L. Trudeau Gene expression profiling in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17{alpha}-ethinylestradiol Physiol Genomics, November 21, 2006; 27(3): 328 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Roesner, T. Hankeln, and T. Burmester Hypoxia induces a complex response of globin expression in zebrafish (Danio rerio) J. Exp. Biol., June 1, 2006; 209(11): 2129 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. M. van der Meer, G. E. E. J. M. van den Thillart, F. Witte, M. A. G. de Bakker, J. Besser, M. K. Richardson, H. P. Spaink, J. T. D. Leito, and C. P. Bagowski Gene expression profiling of the long-term adaptive response to hypoxia in the gills of adult zebrafish Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1512 - R1519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sarropoulou, G. Kotoulas, D. M. Power, and R. Geisler Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology Physiol Genomics, October 17, 2005; 23(2): 182 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nikinmaa and B. B. Rees Oxygen-dependent gene expression in fishes Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1079 - R1090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H. Gibbons, C. C. Liew, M. O. Goodarzi, J. I. Rotter, W. A. Hsueh, H. M. Siragy, R. Pratt, and V. J. Dzau Genetic Markers: Progress and Potential for Cardiovascular Disease Circulation, June 29, 2004; 109(25_suppl_1): IV-47 - IV-58. [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Prasch, E. A. Andreasen, R. E. Peterson, and W. Heideman Interactions between 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and Hypoxia Signaling Pathways in Zebrafish: Hypoxia Decreases Responses to TCDD in Zebrafish Embryos Toxicol. Sci., March 1, 2004; 78(1): 68 - 77. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
