| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Thermal and Mountain Medicine Division, United States Army Research Institute of Environmental Medicine, Natick 01760
2 Division of Pulmonary and Critical Care Medicine
3 Cardiology Division and the Gene Array Technology Center, Brigham and Women’s Hospital/Harvard Medical School, Boston, Massachusetts 02115
The full extent to which hypoxia produces gene expression changes in human cells is unknown. We used late-generation oligonucleotide arrays to catalog hypoxia-induced changes in gene expression in HepG2 cells. Five paired sets of cultures were subjected to either control (room air-5% CO2) or hypoxic (1% O2-5% CO2) conditions for 24 h, and RNA was analyzed on an Affymetrix cDNA array containing 
12,600 sequences. A statistically significant change in expression was shown by 2,908 sequences (1,255 increased and 1,653 decreased). The observed changes were highly concordant with published literature on hypoxic stress but showed relatively little overlap (12–22%) with changes in gene expression that have been reported to occur after heat stress in other systems. Of note, of these 2,908 sequences, only 387 (213 increased and 174 decreased) both exhibited changes in expression of twofold or greater and were highly expressed in at least three of the five experiments. We conclude that the effect of hypoxia on gene expression by HepG2 cells is broad, has a significant component of downregulation, and includes a relatively small number of genes whose response is truly independent of cell and stress type.
cDNA array; hypoxic stress; cellular hypoxia; heat shock; cell stress response
This article has been cited by other articles:
|
|
 |
|
  M. J. Liguori, E. A.G. Blomme, and J. F. Waring Trovafloxacin-Induced Gene Expression Changes in Liver-Derived in Vitro Systems: Comparison of Primary Human Hepatocytes to HepG2 Cells Drug Metab. Dispos., February 1, 2008; 36(2): 223 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Thomas and G. J. Johannes Identification of mRNAs that continue to associate with polysomes during hypoxia RNA, July 1, 2007; 13(7): 1116 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sonna, M. M. Kuhlmeier, H. C. Carter, J. D. Hasday, C. M. Lilly, and K. D. Fairchild Effect of moderate hypothermia on gene expression by THP-1 cells: a DNA microarray study Physiol Genomics, September 14, 2006; 26(1): 91 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Schweppe, T. H. Cheung, and N. G. Ahn Global Gene Expression Analysis of ERK5 and ERK1/2 Signaling Reveals a Role for HIF-1 in ERK5-mediated Responses J. Biol. Chem., July 28, 2006; 281(30): 20993 - 21003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Luyendyk, L. D. Lehman-McKeeman, D. M. Nelson, V. M. Bhaskaran, T. P. Reilly, B. D. Car, G. H. Cantor, X. Deng, J. F. Maddox, P. E. Ganey, et al. Coagulation-Dependent Gene Expression and Liver Injury in Rats Given Lipopolysaccharide with Ranitidine but Not with Famotidine J. Pharmacol. Exp. Ther., May 1, 2006; 317(2): 635 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vengellur, J. M. Phillips, J. B. Hogenesch, and J. J. LaPres Gene expression profiling of hypoxia signaling in human hepatocellular carcinoma cells Physiol Genomics, August 11, 2005; 22(3): 308 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Wang, D. A. Davis, M. Haque, L. E. Huang, and R. Yarchoan Differential Gene Up-Regulation by Hypoxia-Inducible Factor-1{alpha} and Hypoxia-Inducible Factor-2{alpha} in HEK293T Cells Cancer Res., April 15, 2005; 65(8): 3299 - 3306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Lilly, H. Tateno, T. Oguma, E. Israel, and L. A. Sonna Effects of Allergen Challenge on Airway Epithelial Cell Gene Expression Am. J. Respir. Crit. Care Med., March 15, 2005; 171(6): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fang, D. Choi, R. P. Searles, and W. D. Mathers A Time Course Microarray Study of Gene Expression in the Mouse Lacrimal Gland after Acute Corneal Trauma Invest. Ophthalmol. Vis. Sci., February 1, 2005; 46(2): 461 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. N. Zelko and R. J. Folz Extracellular Superoxide Dismutase Functions as a Major Repressor of Hypoxia-Induced Erythropoietin Gene Expression Endocrinology, January 1, 2005; 146(1): 332 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Horowitz, L. Eli-Berchoer, I. Wapinski, N. Friedman, and E. Kodesh Stress-related genomic responses during the course of heat acclimation and its association with ischemic-reperfusion cross-tolerance J Appl Physiol, October 1, 2004; 97(4): 1496 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ning, T. J. Chu, C. J. Li, A. M. K. Choi, and D. G. Peters Genome-wide analysis of the endothelial transcriptome under short-term chronic hypoxia Physiol Genomics, June 17, 2004; 18(1): 70 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Sonna, C. B. Wenger, S. Flinn, H. K. Sheldon, M. N. Sawka, and C. M. Lilly Exertional heat injury and gene expression changes: a DNA microarray analysis study J Appl Physiol, May 1, 2004; 96(5): 1943 - 1953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Wood, L. Tchack, G. Angelo, R. E. Pratt, and L. A. Sonna DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line Physiol Genomics, April 13, 2004; 17(2): 122 - 129. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
