Physiol. Genomics Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 12: 25-33, 2002. First published October 29, 2002; doi:10.1152/physiolgenomics.00016.2002
1094-8341/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
12/1/25    most recent
00016.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peters, D. G.
Right arrow Articles by Ferrell, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peters, D. G.
Right arrow Articles by Ferrell, R. E.
Received 13 February 2002; accepted in final form 20 October 2002.
Physiological Genomics 12:25-33 (2002)
1094-8341/02 $5.00 © 2002 American Physiological Society

Genomic analysis of immediate/early response to shear stress in human coronary artery endothelial cells

D. G. Peters1,3, X.-C. Zhang1, P. V. Benos1,2,3, E. Heidrich-O’Hare1 and R. E. Ferrell1,3

1 Departments of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
2 Center for Computational Biology and Bioinformatics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
3 University of Pittsburgh Cancer Institute, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

The involvement of shear stress in the pathogenesis of vascular disease has motivated efforts to define the endothelial cell response to applied shear stress in vitro. A central question has been the mechanisms by which endothelial cells perceive and respond to changes in fluid flow. We have utilized cDNA microarrays to characterize the immediate/early genomic response to applied laminar shear stress (LSS) in primary cultures of human coronary artery endothelial cells (HCAECs). Cells were exposed, in a parallel plate flow chamber, to 0, 15, or 45 dyn/cm2 LSS for 1 h, and gene expression profiles were determined using human GEM1 cDNA microarrays. We find that a high proportion of LSS-responsive genes are transcription factors, and these are related by their involvement in growth arrest. These likely play a central role in the reprogramming of endothelial homeostasis following the switch from a static to a shear-stressed environment. LSS-responsive genes were also found to encode factors involved in vasoreactivity, signal transduction, antioxidants, cell cycle-associated genes, and markers of cytoskeletal function and dynamics.

gene expression; atherosclerosis; endothelial cell; microarray




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. D. Ochoa, H. Baker, S. Hasak, R. Matyal, A. Salam, C. A. Hales, W. Hancock, and D. A. Quinn
Cyclic Stretch Affects Pulmonary Endothelial Cell Control of Pulmonary Smooth Muscle Cell Growth
Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 105 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
D. L. Corcoran, E. Feingold, J. Dominick, M. Wright, J. Harnaha, M. Trucco, N. Giannoukakis, and P. V. Benos
Footer: A quantitative comparative genomics method for efficient recognition of cis-regulatory elements
Genome Res., June 1, 2005; 15(6): 840 - 847.
[Abstract] [Full Text] [PDF]

Home page
 VASC ENDOVASCULAR SURGHome page
J. L. Unthank, K. M. Sheridan, and M. C. Dalsing
Collateral Growth in the Peripheral Circulation: A Review
Vascular and Endovascular Surgery, July 1, 2004; 38(4): 291 - 313.
[Abstract] [PDF]

Home page
 Physiol. GenomicsHome page
P. Yang, O. Agapova, A. Parker, W. Shannon, P. Pecen, J. Duncan, M. Salvador-Silva, and M. R. Hernandez
DNA microarray analysis of gene expression in human optic nerve head astrocytes in response to hydrostatic pressure
Physiol Genomics, April 13, 2004; 17(2): 157 - 169.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kondapalli, A. S. Flozak, and M. L. C. Albuquerque
Laminar Shear Stress Differentially Modulates Gene Expression of p120 Catenin, Kaiso Transcription Factor, and Vascular Endothelial Cadherin in Human Coronary Artery Endothelial Cells
J. Biol. Chem., March 19, 2004; 279(12): 11417 - 11424.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. G. Passerini, D. C. Polacek, C. Shi, N. M. Francesco, E. Manduchi, G. R. Grant, W. F. Pritchard, S. Powell, G. Y. Chang, C. J. Stoeckert Jr., et al.
Coexisting proinflammatory and antioxidative endothelial transcription profiles in a disturbed flow region of the adult porcine aorta
PNAS, February 24, 2004; 101(8): 2482 - 2487.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online