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Physiol. Genomics 1: 71-73, 1999. First published August 31, 1999;
1094-8341/99 $5.00
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Received 10 March 1999; accepted in final form 29 July 1999.
Physiological Genomics 1:71-73 (1999)
1094-8341/99 $5.00 © 1999 American Physiological Society

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A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism

CLIVE C. J. HUNT, JODI E. BURLEY, CAROLINE M. L. CHAPMAN and JOHN P. BEILBY

The Western Australian Centre for Pathology and Medical Research (PathCentre), Perth, Australia

Hunt, Clive C. J., Jodi E. Burley, Caroline M. L. Chapman, and John P. Beilby. A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism. Physiol. Genomics 1: 71–73, 1999.—We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT1) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT1 A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.

receptors; angiotensin II type-1 receptor; mutagenically separated polymerase chain reaction; microtiter array diagonal gel electrophoresis; polymorphism; genetics




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